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Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Suppressor of Fused.
(Peptide available as ab28933.)
Our Abpromise guarantee covers the use of ab28083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Suppressor of Fused knockout HAP1 cell lysate (20 µg)
Lane 3: LnCaP cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab28083 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab28083 was shown to recognize Suppressor of Fused when Suppressor of Fused knockout samples were used, along with additional cross-reactive bands. Wild-type and Suppressor of Fused knockout samples were subjected to SDS-PAGE. ab28083 and ab8245 (loading control to GAPDH) were both diluted at 1 µg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.