Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)


  • 製品名Superoxide Dismutase Activity Assay Kit (Colorimetric)
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプEnzyme activity
  • 全工程の試験時間
    0h 30m
  • 種交差性
    交差種: Mouse, Rat, Sheep, Human, Pig
    交差が予測される動物種: Mammal
  • 製品の概要

    Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen.

  • アプリケーション適用あり: Functional Studiesmore details


  • 関連性Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
  • 細胞内局在Cytoplasmic
  • 別名
    • ALS
    • ALS1
    • Cu /Zn superoxide dismutase
    • Cu/Zn superoxide dismutase
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase cystolic
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab65354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Superoxide Dismutase Activity Assay Kit (Colorimetric) 画像

  • Superoxide dismutase measured in biofluids at various dilutions

  • Superoxidase dismutase (ab90040) measured showing inhibition rate (%) per concentration (microgram per mL)

  • Principle of Superoxide Dismutase Assay.


Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Liquiritigenin Protects Rats from Carbon Tetrachloride Induced Hepatic Injury through PGC-1a Pathway. Evid Based Complement Alternat Med 2015:649568 (2015). Functional Studies . Read more (PubMed: 26199636) »
  • Chade AR  et al. Disparate effects of single endothelin-A and -B receptor blocker therapy on the progression of renal injury in advanced renovascular disease. Kidney Int 85:833-44 (2014). Read more (PubMed: 24352153) »

See all 10 Publications for this product

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I would not suggest this, as we store this product at 4C ourselves. We haven't tried to store this productat -20C, and we do not know if the product is suitable to be stored at lower temperatures. Please be aware...

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The ab65354 Superoxide Dismutase Activity Colorimetric Assay Kit can be used without a standard since it is reporting as % inhibition in your sample. Blank 1 does not contain any inhibition, and your sample will have some percentage of inhibition. Bla...

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I apologize for the problems you had with accessing our website.

As you had requested, I have attached the SDS for the 3 of the kits you mentioned.

The links to the online SDSpages are also: ...

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To our knowledge, ab65354 has not been tested specifically for use with drosophila samples. This assay, however, is not species specific. So long as the SOD present in your drosophila samples is inhibiting the red...

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Regarding the ab112193 Recombinant Superoxide Dismutase 1 protein standard for use with ab65354 Superoxide Dismutase Activity Assay Kit:  The concentration of Cu and Zn have not been measured as they are bound to the protein and come from fermenta...

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Thank you for your enquiry.

I appreciate your patience. It has taken some time to confirm details with the laboratories.

This kit is not originally designed to be run with a standard curve each time. However, if you wish to run a stan...

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I can confirm that there is nothing isoform-specific in the kit principle. ab65354 measures the oxygen anion created by any of the SOD enzymes.

The minimum mass of tissue to use with this assay is 10mg.

Thank you for contacting us.

This kit has been tested in human samples only, so it may work for prokaryotic cells but we have no data on testing these types of samples.

I hope this information is helpful to you. Please do not hesitate...

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We would recommend using at least 10 mg of tissue with the kit ab65354.

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