Anti-Superoxide Dismutase 4 抗体 [2A1] (ab16963)

製品の概要

  • 製品名Anti-Superoxide Dismutase 4 antibody [2A1]
    Superoxide Dismutase 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A1] to Superoxide Dismutase 4
  • アプリケーション適用あり: ICC/IF, ELISA, IPmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能Delivers copper to copper zinc superoxide dismutase (SOD1).
  • 組織特異性Ubiquitous.
  • 配列類似性In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.
    Contains 1 HMA domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCS antibody
    • CCS_HUMAN antibody
    • Copper chaperone for superoxide dismutase antibody
    • MGC138260 antibody
    • SOD 4 antibody
    • SOD4 antibody
    • Superoxide dismutase copper chaperone antibody
    see all

Anti-Superoxide Dismutase 4 antibody [2A1] 画像

  • ab16963 staining Superoxide Dismutse 4 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Immunoprecipitation analysis of HeLa cell lysates using ab16963.
    Lane 1- Input
    Lane 2- IP

    Western analysis was conducted using a polyclonal SOD4 antibody (ab16834)

Anti-Superoxide Dismutase 4 antibody [2A1] (ab16963) 使用論文

ab16963 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"