製品の概要

  • 製品名Anti-Superoxide Dismutase 3 antibody
    Superoxide Dismutase 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Superoxide Dismutase 3
  • 特異性Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.
  • アプリケーション適用あり: IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    WTGEDSAEPNSDSAEWIRD

    , corresponding to N terminal amino acids 19-37 of Human Superoxide Dismutase 3.

  • ポジティブ・コントロール
    • IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200 - 1/2000.
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).

ターゲット情報

  • 機能Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • 組織特異性Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • 配列類似性Belongs to the Cu-Zn superoxide dismutase family.
  • 細胞内局在Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC SOD antibody
    • EC-SOD antibody
    • Extracellular superoxide dismutase [Cu Zn] antibody
    • Extracellular superoxide dismutase [Cu-Zn] antibody
    • Extracellular superoxide dismutase antibody
    • Extracellular superoxide dismutase precursor antibody
    • MGC20077 antibody
    • SOD 3 antibody
    • SOD3 antibody
    • SODE_HUMAN antibody
    • Superoxide dismutase 3 extracellular antibody
    see all

Anti-Superoxide Dismutase 3 antibody 画像

  • ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
  • ab21974 (1/1000) staining of human kidney tubule cells.
  • All lanes : Anti-Superoxide Dismutase 3 antibody (ab21974) at 1/500 dilution

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-Superoxide Dismutase 3 antibody (ab21974) 使用論文

This product has been referenced in:
  • Duscher D  et al. Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells. Sci Rep 4:7144 (2014). IHC-Fr ; Mouse . Read more (PubMed: 25413454) »
  • Cunningham MW  et al. Renal nitric oxide synthase and antioxidant preservation in cyp1a1-ren-2 transgenic rats with inducible malignant hypertension. Am J Hypertens 26:1242-9 (2013). Read more (PubMed: 23764378) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate Buffer + 0.05% Tween 20
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Formaldehyde
Username

Dr. I Zelko

Verified customer

投稿 Jan 25 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Penile Tissue)
Loading amount 30 µg
Specification Mouse Penile Tissue
Gel Running Conditions Reduced Denaturing (4-15% minigel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

投稿 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"