Anti-Superoxide Dismutase 3 抗体 [1H12] (ab28442)
製品の概要
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製品名Anti-Superoxide Dismutase 3 antibody [1H12]
Superoxide Dismutase 3 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [1H12] to Superoxide Dismutase 3
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アプリケーション適用あり: WB, IP, IHC-P, ChIP, ELISAmore details
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種交差性交差種: Human
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免疫原
Recombinant full length protein (Human) purified from E.coli.
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ポジティブ･コントロール
- Bosc23 cell lysate transfected with Myc-His-SOD3.
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特記事項
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR58884 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名1H12
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アイソタイプIgG2b
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab28442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa. | |
| IP | Use at an assay dependent concentration. Use 2μg |
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| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ChIP | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
ターゲット情報
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機能Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
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組織特異性Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
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配列類似性Belongs to the Cu-Zn superoxide dismutase family.
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細胞内局在Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
- Information by UniProt
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参照データベース
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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別名
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
Anti-Superoxide Dismutase 3 antibody [1H12] 画像
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![Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)](http://a.abcam.com/ps/datasheet/Images/28/ab28442/Ab28442_1.JPG)
Western blot - Superoxide Dismutase 3 antibody [1H12] (ab28442)All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
Lane 1 : Bosc23 cell lysate
Lane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3
Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3
Predicted band size : 26 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?) -
](http://a.abcam.com/ps/datasheet/images/28/ab28442/Superoxide-Dismutase-3-Primary-antibodies-ab28442-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Superoxide Dismutase 3 antibody [1H12](ab28442)IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442) 使用論文
This product has been referenced in:
- Nightingale H et al. Changes in Expression of the Antioxidant Enzyme SOD3 Occur Upon Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells In Vitro. Stem Cells Dev : (2012). Read more (PubMed: 22132904) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"