製品の概要

  • 製品名
    Anti-Superoxide Dismutase 3 antibody [1H12]
    Superoxide Dismutase 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H12] to Superoxide Dismutase 3
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IP, IHC-P, ChIP, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human) purified from E.coli.

  • ポジティブ･コントロール
    • Bosc23 cell lysate transfected with Myc-His-SOD3.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR58884 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.

Use 2μg
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • 組織特異性
    Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.
  • 配列類似性
    Belongs to the Cu-Zn superoxide dismutase family.
  • 細胞内局在
    Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.
  • Information by UniProt
  • 参照データベース
    • 別名
      • EC SOD antibody
      • EC-SOD antibody
      • Extracellular superoxide dismutase [Cu Zn] antibody
      • Extracellular superoxide dismutase [Cu-Zn] antibody
      • Extracellular superoxide dismutase antibody
      • Extracellular superoxide dismutase precursor antibody
      • MGC20077 antibody
      • SOD 3 antibody
      • SOD3 antibody
      • SODE_HUMAN antibody
      • Superoxide dismutase 3 extracellular antibody
      see all

    画像

    • Western blot - Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
      Western blot - Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
      All lanes : Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)

      Lane 1 : Bosc23 cell lysate
      Lane 2 : Bosc23 cell lysate transfected with Myc-His-SOD3
      Lane 3 : Bosc23 cell lysate transfected with Myc-His-Gpx3

      Predicted band size: 26 kDa
      Observed band size: 35 kDa (why is the actual band size different from the predicted?)



      The arrow next to the image points to the Superoxide Dismutase 3 band.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3 antibody [1H12] (ab28442)
      IHC image of ab28442 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28442, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    This product has been referenced in:
    • Nightingale H  et al. Changes in Expression of the Antioxidant Enzyme SOD3 Occur Upon Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells In Vitro. Stem Cells Dev : (2012). Read more (PubMed: 22132904) »

    See 1 Publication for this product

    ab28442 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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