Anti-Superoxide Dismutase 1 抗体 (ab52950)


  • 製品名
    Anti-Superoxide Dismutase 1 antibody
    Superoxide Dismutase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Superoxide Dismutase 1
  • 由来種
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human Superoxide Dismutase 1

  • ポジティブ・コントロール
    • Jurkat cell lysate, human placenta and HeLa cells.



Our Abpromise guarantee covers the use of ab52950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/20.
IHC-P 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • 関連疾患
      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • 配列類似性
      Belongs to the Cu-Zn superoxide dismutase family.
    • 翻訳後修飾
      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • 細胞内局在
      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all


    • Anti-Superoxide Dismutase 1 antibody (ab52950) at 1/5000 dilution + Jurkat cell lysate at 10 µg

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa

    • ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in human placenta by Immunohistochemistry, Paraffin embedded tissue.


    This product has been referenced in:
    • Shvil N  et al. MIF inhibits the formation and toxicity of misfolded SOD1 amyloid aggregates: implications for familial ALS. Cell Death Dis 9:107 (2018). WB . Read more (PubMed: 29371591) »
    • Jiang HZ  et al. Downregulation of Homer1b/c in SOD1 G93A Models of ALS: A Novel Mechanism of Neuroprotective Effect of Lithium and Valproic Acid. Int J Mol Sci 17:N/A (2016). WB . Read more (PubMed: 27999308) »

    See all 13 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Western blot
    Bos taurus Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    20 µg
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Julie Kim

    Verified customer

    投稿 May 12 2017

    Our cross reactivity recommendations for 1926-1 and 2582-1 are based off of Western blot analysis only. We do not recommend either product for detection of mouse SOD1 as we did not obtain any staining in WB with 2582-1, and we obtained non-specifi...

    Read More

    Vielen Dank für Ihre Antwort.

    Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu...

    Read More

    Vielen Dank für Ihre Anfrage.
    Ich freue mich Ihnen mitteilen zu können, dass beide Lots eine Konzentration von 0.263 mg/mL haben.
    Ich möchte auch darauf hinweisen, dass diese scheinbar niedrige Konzentration völlig ausreichend ist. Dieser Ant...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
    Loading amount
    15 µg
    1 EBNA, 2 HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%

    Abcam user community

    Verified customer

    投稿 Dec 16 2008