Anti-Superoxide Dismutase 1 抗体 (ab52950)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Superoxide Dismutase 1 antibody
Superoxide Dismutase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Superoxide Dismutase 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Jurkat cell lysate, human placenta and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52950の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
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特記事項 |
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WB
1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/50 - 1/100. Antigen retrieval is not essential but may optimise staining. |
ターゲット情報
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機能
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
配列類似性
Belongs to the Cu-Zn superoxide dismutase family. -
翻訳後修飾
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
細胞内局在
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
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参照データベース
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00441 Human
- Unigene: 443914 Human
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別名
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
画像
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Anti-Superoxide Dismutase 1 antibody (ab52950) at 1/5000 dilution + Jurkat cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
ab52950 at 1/50 dilution staining Superoxide Dismutase 1 in human placenta by Immunohistochemistry, Paraffin embedded tissue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab52950 は 21 報の論文で使用されています。
- Peng J et al. MPO/HOCl Facilitates Apoptosis and Ferroptosis in the SOD1G93A Motor Neuron of Amyotrophic Lateral Sclerosis. Oxid Med Cell Longev 2022:8217663 (2022). PubMed: 35178161
- Biyik I et al. The effects of recombinant klotho in cisplatin-induced ovarian failure in mice. J Obstet Gynaecol Res 47:1817-1824 (2021). PubMed: 33611838
- Chu Q et al. Studies on the Neuroprotection of Osthole on Glutamate-Induced Apoptotic Cells and an Alzheimer's Disease Mouse Model via Modulation Oxidative Stress. Appl Biochem Biotechnol 190:634-644 (2020). PubMed: 31407160
- Gill C et al. SOD1-positive aggregate accumulation in the CNS predicts slower disease progression and increased longevity in a mutant SOD1 mouse model of ALS. Sci Rep 9:6724 (2019). PubMed: 31040321
- Shvil N et al. MIF inhibits the formation and toxicity of misfolded SOD1 amyloid aggregates: implications for familial ALS. Cell Death Dis 9:107 (2018). WB . PubMed: 29371591