Anti-Superoxide Dismutase 1 抗体 (ab16831)

製品の概要

  • 製品名Anti-Superoxide Dismutase 1 antibody
    Superoxide Dismutase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Superoxide Dismutase 1
  • 特異性There is no cross-reactivity with SOD2,3 and 4 by WB.
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque Monkey
  • 免疫原

    Recombinant full length protein (Human) purified from E Coli

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml. Supplier new lot doesn't work in IP
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 配列類似性Belongs to the Cu-Zn superoxide dismutase family.
  • 翻訳後修飾Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 細胞内局在Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

Anti-Superoxide Dismutase 1 antibody 画像



  • Predicted band size : 17 kDa

    ab16831 at 1/2000 detecting Superoxide Dismutase 1 by Western blot

    Lane 1: Mouse brain

    Lane 2: Mouse liver

    Lane 3: HeLa cell lysate

    Lane 4: 293T cell lysate

    Lane 5: Jurkat  cell lysate

    ab16831 at 1/2000 detecting Superoxide Dismutase 1 by Western blot

    Lane 1: Mouse brain

    Lane 2: Mouse liver

    Lane 3: HeLa cell lysate

    Lane 4: 293T cell lysate

    Lane 5: Jurkat cell lysate

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution

    Lane 1 : 40ug mouse liver homogenate
    Lane 2 : 20ug mouse liver homogenate
    Lane 3 : 5ug mouse liver homogenate

    Secondary
    HRP conjugated donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa


    Exposure time : 20 seconds
    This image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.

    See Abreview

  • Ab16831 staining human normal placenta tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab16831 staining superoxide dismutase in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.

    See Abreview

  • Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution + Recombinant human Superoxide Dismutase 1 protein (ab90040) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa


    Exposure time : 4 minutes

Anti-Superoxide Dismutase 1 antibody (ab16831) 使用論文

This product has been referenced in:
  • Greco T  et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016). Read more (PubMed: 26661201) »
  • Tsang CK  et al. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance. Nat Commun 5:3446 (2014). Read more (PubMed: 24647101) »

See all 27 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Placenta)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Placenta
Blocking step BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vicky

Verified customer

投稿 Nov 29 2016

Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Human fibroblasts
Blocking step Licor odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 25 2016

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Jurkats)
Specification Jurkats
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

投稿 Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

Vielen Dank für Ihre Antwort.

Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Isolated Lung)
Loading amount 20 µg
Specification Isolated Lung
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Sep 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Tubules (Kidney))
Specification Tubules (Kidney)
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (bone marrow cells)
Specification bone marrow cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 20 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 28 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"