Anti-Superoxide Dismutase 1 抗体 (ab16831)

製品の概要

  • 製品名
    Anti-Superoxide Dismutase 1 antibody
    Superoxide Dismutase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Superoxide Dismutase 1
  • 特異性
    There is no cross-reactivity with SOD2,3 and 4 by WB.
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-P, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque monkey
  • 免疫原

    Recombinant full length protein corresponding to Human Superoxide Dismutase 1.
    Database link: P00441

  • ポジティブ・コントロール
    • WB: Mouse brain and mouse liver tissue lysates, HeLa, 293T and Jurkat cell lysates. IHC-P: Human placenta tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 配列類似性
    Belongs to the Cu-Zn superoxide dismutase family.
  • 翻訳後修飾
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 細胞内局在
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

画像

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Jurkat cell lysate


    Predicted band size : 17 kDa
  • ab16831 staining human normal placenta tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab16831 staining Superoxide Dismutase 1 in rat bone marrow cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 2 hours at 25°C. Samples were incubated with the primary antibody (1/250 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. DAPI used to stain the nucleus.

    See Abreview

  • ab16831 staining superoxide dismutase in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.

    See Abreview

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution

    Lane 1 : 40ug mouse liver homogenate
    Lane 2 : 20ug mouse liver homogenate
    Lane 3 : 5ug mouse liver homogenate

    Secondary
    HRP conjugated donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa


    Exposure time : 20 secondsThis image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.

    See Abreview

  • Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution + Recombinant human Superoxide Dismutase 1 protein (ab90040) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa


    Exposure time : 4 minutes

参考文献

This product has been referenced in:
  • Bodega G  et al. The Antioxidant Machinery of Young and Senescent Human Umbilical Vein Endothelial Cells and Their Microvesicles. Oxid Med Cell Longev 2017:7094781 (2017). WB ; Human . Read more (PubMed: 28642812) »
  • Kaczor JJ  et al. Higher oxidative stress in skeletal muscle of McArdle disease patients. Mol Genet Metab Rep 12:69-75 (2017). WB ; Human . Read more (PubMed: 28649515) »

See all 42 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Placenta
Blocking step
BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vicky

Verified customer

投稿 Nov 29 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Human fibroblasts
Blocking step
Licor odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 25 2016

Application
Western blot
Loading amount
20000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Jurkats)
Specification
Jurkats
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

投稿 Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

Vielen Dank für Ihre Antwort.

Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Isolated Lung)
Loading amount
20 µg
Specification
Isolated Lung
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Sep 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Tubules (Kidney))
Specification
Tubules (Kidney)
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (bone marrow cells)
Specification
bone marrow cells
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
20 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 28 2010

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録