製品の概要

  • 製品名
    Anti-Superoxide Dismutase 1 antibody
    Superoxide Dismutase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Superoxide Dismutase 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, WB, IP, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey, African green monkey
  • 免疫原

    Full length protein corresponding to Human Superoxide Dismutase 1.

  • ポジティブ・コントロール
    • IHC-P: Human placenta tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000. See Abreview.
IHC-Fr 1/1000.
WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.
IP Use a concentration of 10 µg/ml. See Shinder et al 2001.
ELISA Use at an assay dependent concentration. See Downs et al 2002.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 配列類似性
    Belongs to the Cu-Zn superoxide dismutase family.
  • 翻訳後修飾
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 細胞内局在
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all

画像

  • Anti-Superoxide Dismutase 1 antibody (ab13498) at 1/5000 dilution + Whole rat tissue lysate at 20 µg

    Secondary
    HRP-conjugated donkey anti-rabbit polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa


    Exposure time: 60 seconds


    Blocking: 5% BSA for 1 hour at 20o.

    See Abreview

  • All lanes : Anti-IL18 antibody (ab71495) at 1/1000 dilution

    All lanes : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 15 kDa (why is the actual band size different from the predicted?)


    Exposure time: 140 seconds


    Blocked with 3% milk for 2 hours at 25°C.

    Incubated with the primary antibody for 16 hours at 4°C in 3% milk in TBS-Tween (0.005%).

    See Abreview

  • ab71495 staining Superoxide Dismutase 1 in cow aorta endothelial cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% saponin and blocked with PBS + 0.1% saponin + 1% BSA for 1 hour at 24°C. Samples were incubated with the primary antibody (1/200 in PBS + 0.1% saponin + 1% BSA) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody at a dilution of 1/300.

    See Abreview

  • ab13498 at 1µg/ml staining Superoxide dismutase 1 in human placenta tissue section by Immunohistochemistry ((Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti-rabbit secondary was used at 1/10 dilution.

  • ab13498 staining Superoxide Dismutase 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

  • ab13498 (1/200) staining Human cell line 293FT by ICC/IF. The 293 FT cells were cultured for 3 days, fixed in 3.7% formaldehyde and blocked with 5% BSA in PBS for 1 hr. The secondary antibody was goat anti-Rabbit IgG conjugated to Alexa Fluor® 488 (green) and the nucleus (blue) were stained with DAPI.

    See Abreview

  • ab13498 staining Superoxide Dismutase 1 in mouse bone marrow white blood cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 12 hours at 4°C. The secondary antibody used was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (polyclonal), diluted to 1/500.
    Nuclei were counterstained blue with DAPI.

    See Abreview

参考文献

This product has been referenced in:

See all 44 Publications for this product

レビューと Q&A

Filter by Application

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Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Feb 28 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Permeabilization
No
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

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投稿 Nov 07 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (retina)
Gel Running Conditions
Non-reduced Denaturing (10% TGX gel)
Loading amount
30 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain 20um thick)
Permeabilization
Yes - 0.1% triton-x
Specification
Brain 20um thick
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Kelly Maers

Verified customer

投稿 Jan 24 2017

Application
Western blot
Sample
Silk worm (Bombyx mori) Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
Gel Running Conditions
Non-reduced Denaturing (12% polyacrylamide gel)
Loading amount
10 µg
Specification
testis lysate (silkworm), BmN4 cell lysate (silkwo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

陽水 野島

Verified customer

投稿 Aug 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (aorta endothelial cells)
Permeabilization
Yes - Saponin 0,1%
Specification
aorta endothelial cells
Blocking step
PBS/Saponin0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
PFA 4%
Username

Miss. Virginie Joris

Verified customer

投稿 Jun 30 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15% Gel)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15% Gel)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (hypocamp foetal)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
20 µg
Specification
hypocamp foetal
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount
30 µg
Specification
Skeletal muscle
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 32°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Jan 06 2012

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