The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Suggested method: place sample in 1X citrate buffer pH 6.0, microwave at 750W for 20 minutes, let sample cool down.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Energy metabolism; sulfur metabolism.
Defects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD) [MIM:272300]; also known as sulfocysteinuria. ISOD is characterized by neurological abnormalities including multicystic leukoencephalopathy with brain atrophy. Patients often suffer from seizures. Often leads to death at an early age.
Immunoperoxidase staining of SUOX in formalin-fixed, paraffin-embedded Human kidney using purified ab88346 at 3 µg/ml. Antigen retrieval was performed by placing sample in 1X citrate buffer pH 6.0 and microwaving at 750W for 20 minutes.