製品の概要

  • 製品名Anti-SUN1 antibody [EPR6554]
    SUN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6554] to SUN1
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to residues in Human SUN1.

  • ポジティブ・コントロール
    • Ramos, K562, HeLa, Jurkat and A431 cell lysates; Human ovary carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs).
    • 配列類似性Contains 1 SUN domain.
    • ドメインThe SUN domain may play a role in the nuclear anchoring and/or migration.
    • 細胞内局在Nucleus inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Protein unc-84 homolog A antibody
      • Sad1 and UNC84 domain containing 1 antibody
      • Sad1/unc 84 protein like 1 antibody
      • Sad1/unc-84 protein-like 1 antibody
      • Sun domain-containing protein 1 antibody
      • Sun1 antibody
      • SUN1_HUMAN antibody
      • UNC 84 homolog A (C elegans) antibody
      • UNC 84A antibody
      • UNC84 A antibody
      • UNC84 antibody
      • UNC84 homolog A (C elegans) antibody
      • UNC84A antibody
      see all

    Anti-SUN1 antibody [EPR6554] 画像



    • Performed under reducing conditions.

      Predicted band size : 90 kDa


      Exposure time : 7 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    • All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 90 kDa
    • ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

    Anti-SUN1 antibody [EPR6554] (ab124770) 使用論文

    This product has been referenced in:
    • Chow KH  et al. The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Mol Biol Cell 25:160-8 (2014). Human . Read more (PubMed: 24196834) »

    See 1 Publication for this product

    Product Wall

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 36 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (HeLa, RPE-1)
    Specification HeLa, RPE-1
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 May 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (skin)
    Loading amount 10 µg
    Specification skin
    Treatment SiRNA SUN1for 48h
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS)
    Loading amount 20 µg
    Specification U2OS
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Andrew Cobb

    Verified customer

    投稿 Jul 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"