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This antibody recognizes Human Sumo 2 and Sumo 3.
This antibody shows no cross-reactivity with related proteins SUMO1, UCRP, Nedd8, and FAT10 in Western blotting.
MSEEKPKEGVKTEND, corresponding to N terminal amino acids 1-15 of Human Sumo 2.
Our Abpromise guarantee covers the use of ab22654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa). Dilute to working strength with 50mM sodium phosphate buffer (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).|