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Our Abpromise guarantee covers the use of ab11672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21317883|
|WB||1/1000 - 1/5000. Predicted molecular weight: 12 kDa. For 1 hour 30 minutes at RT in 5% milk in TBST (note: 0.7% Tween).
A 1/5000 dilution of the antibody will detect 0.1ng of the recombinant protein used as an immunogen.
|IHC-P||1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
Immunofluorescence of 293T cells transfected with a vector that has Sumo1 fused to GFP and a Flag tag. The top panel is GFP fluorescence. The middle panel uses ab11672 at 1/300 with a rhodamine secondary antibody. The lower panel is a merge of the GFP fluorescence and ab11672 immunostaining. Staining was also seen in untransfected cells (although at a lower level).
293T cells were transfected with a vector that has Sumo1 fused to GFP and a Flag tag. Cell lysates were used in IP with ab11672 (and a GFP antibody as a control). The resulting western blot was performed with a Flag antibody. As a control, cells were transfected with a a vector with Sumo2 fused to GFP and a Flag tag. ab11672 does not IP anything from this lysate.
Lane 1: Sumo1 fusion lysate - IP'd with GFP antibody
Lane 2: Sumo1 fusion lysate - no IP
Lane 3: Sumo1 fusion lysate - IP'd with ab11672
Lane 4: Sumo2 fusion lysate - IP'd with ab11672