製品の概要

  • 製品名Anti-Sumo 1 antibody
    Sumo 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sumo 1
  • 特異性This antibody is react to human Sumo 1 and does not cross react with ubiquitin.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide:

    HAKPSTEDLGDKKEGEY

    , corresponding to amino acids 6-21 of Human Sumo 1. The peptide was coupled to Diphtheria toxoid via a cysteine residue added at the Carboxyl terminus of the peptide. The amino acid residues 6-21 are identical between human and mouse Sumo 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-P
  • 追加情報IHC-P: 1/1000. Antigen retrieval is not essential but may optimise staining.
    WB: 1/2000. Detects a band of approximately 12 kDa.
    ICC/IF: 1/1000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
    • 関連疾患Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
    • 配列類似性Belongs to the ubiquitin family. SUMO subfamily.
      Contains 1 ubiquitin-like domain.
    • 翻訳後修飾Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
      Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
    • 細胞内局在Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DAP1 antibody
      • GAP modifying protein 1 antibody
      • GAP-modifying protein 1 antibody
      • GMP 1 antibody
      • GMP1 antibody
      • OFC10 antibody
      • PIC 1 antibody
      • PIC1 antibody
      • SENP2 antibody
      • Sentrin 1 antibody
      • Sentrin antibody
      • Small ubiquitin related modifier 1 antibody
      • Small ubiquitin-like modifier 1 antibody
      • Small ubiquitin-related modifier 1 antibody
      • SMT3 antibody
      • SMT3 homolog 3 antibody
      • SMT3 suppressor of mif two 3 homolog 1 antibody
      • SMT3, yeast, homolog 3 antibody
      • Smt3C antibody
      • SMT3H3 antibody
      • SUMO-1 antibody
      • SUMO1 antibody
      • SUMO1_HUMAN antibody
      • Ubiquitin homology domain protein PIC1 antibody
      • Ubiquitin Like 1 antibody
      • Ubiquitin like protein SMT3C antibody
      • Ubiquitin like protein UBL1 antibody
      • Ubiquitin-homology domain protein PIC1 antibody
      • Ubiquitin-like protein SMT3C antibody
      • Ubiquitin-like protein UBL1 antibody
      • UBL 1 antibody
      • UBL1 antibody
      see all

    Anti-Sumo 1 antibody 画像

    • ICC/IF image of ab11291 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11291, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Sumo 1 antibody (ab11291) 使用論文

    This product has been referenced in:
    • Gowran A  et al. Delta9-tetrahydrocannabinol regulates the p53 post-translational modifiers Murine double minute 2 and the Small Ubiquitin MOdifier protein in the rat brain. FEBS Lett 583:3412-8 (2009). WB ; Rat . Read more (PubMed: 19819240) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I apologize since the MW is incorrect on these datasheets. The predicted weight of Sumo 1 based on the Swiss-Prot database as well as testing is 12 kDa. I have corrected the online datasheets. Since sumo 1 is a ubiquit...

    Read More

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