Anti-FGE 抗体 (ab91479)
Key features and details
- Goat polyclonal to FGE
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-FGE antibody -
製品の詳細
Goat polyclonal to FGE -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human FGE aa 314-327 (internal sequence).
Sequence:C-ETLNPKGPPSGKDR
Database link: NP_877437.2 -
ポジティブ・コントロール
- Mouse pancreas, Mouse eye and Human kidney lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab91479 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. -
組織特異性
Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart. -
パスウェイ
Protein modification; sulfatase oxidation. -
関連疾患
Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive. -
配列類似性
Belongs to the sulfatase-modifying factor family. -
翻訳後修飾
N-glycosylated. Contains high-mannose-type oligosaccharides. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 536435 Cow
- Entrez Gene: 484681 Dog
- Entrez Gene: 58911 Mouse
- Entrez Gene: 362409 Rat
- SwissProt: Q0P5L5 Cow
- SwissProt: Q8R0F3 Mouse
- Unigene: 439876 Mouse
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別名
- MGC150436 antibody
- AAPA3037 antibody
- C alpha formylglycine generating enzyme 1 antibody
see all
画像
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Anti-FGE antibody (ab91479) at 1 µg/ml + Mouse Pancreas lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab91479 は論文での使用が確認できていません。