製品の概要

  • 製品名
    Anti-SULT2B1 antibody
  • 製品の詳細
    Rabbit polyclonal to SULT2B1
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (

    YSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFI

    ) of Human SULT2B1 (NP_004596).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay is 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
  • 組織特異性
    Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
  • 配列類似性
    Belongs to the sulfotransferase 1 family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alcohol sulfotransferase antibody
    • EC 2.8.2.2 antibody
    • HSST2 antibody
    • Hydroxysteroid sulfotransferase 2 antibody
    • ST2B1 antibody
    • ST2B1_HUMAN antibody
    • Sulfotransferase 2B1 antibody
    • Sulfotransferase family cytosolic 2B member 1 antibody
    • SULT2B1 antibody
    • SULT2B1a antibody
    • SULT2B1b antibody
    see all

画像

  • Anti-SULT2B1 antibody (ab82865) at 1 µg/ml (5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • Anti-SULT2B1 antibody (ab82865) at 1/1000 dilution + whole cell lysate prepared from murine macrophages at 30 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

参考文献

This product has been referenced in:
  • Segala G  et al. 5,6-Epoxy-cholesterols contribute to the anticancer pharmacology of Tamoxifen in breast cancer cells. Biochem Pharmacol 86:175-89 (2013). Read more (PubMed: 23500540) »
  • Yang X  et al. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo. PLoS One 8:e60853 (2013). WB, ICC/IF ; Mouse, Human . Read more (PubMed: 23593328) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (liver cells)
Specification
liver cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophages)
Loading amount
30 µg
Specification
macrophages
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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