製品の概要

  • 製品名Anti-SULT2A1 antibody
    SULT2A1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SULT2A1
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human SULT2A1 aa 253-285 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
  • 組織特異性Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the adrenal than the liver and the kidney.
  • 配列類似性Belongs to the sulfotransferase 1 family.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alcohol/hydroxysteroid sulfotransferase antibody
    • Bile salt sulfotranasferase 2A1 antibody
    • Bile salt sulfotransferase antibody
    • Dehydroepiandrosterone sulfotransferase antibody
    • DHEA ST antibody
    • DHEA sulfotranasferase antibody
    • DHEA-ST antibody
    • DHEAS antibody
    • EC 2.8.2.14 antibody
    • Hst antibody
    • hSTa antibody
    • Hydroxysteroid sulfotransferase antibody
    • ST2 antibody
    • ST2A1 antibody
    • ST2A1_HUMAN antibody
    • ST2A3 antibody
    • STD antibody
    • sulfotranasferase, dehydroepiandrosterone-preferring antibody
    • Sulfotransferase 2A1 antibody
    • Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 antibody
    • Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 antibody
    • Sult2a1 antibody
    see all

Anti-SULT2A1 antibody 画像

  • Anti-SULT2A1 antibody (ab38416) at 1/100 dilution + Mouse kidney tissue lysate.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa


  • Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Both lanes: SULT2A1 antibody (ab38416) at 1/500 dilution (in PBS + 0.1% Tween20) for 1 hour at 22°C + whole tissue lysate of human liver at 20 µg.

    Secondary
    An HRP conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time: 1 minute 20 seconds

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

  • IHC image of ab38416 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38416, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of Anti-SULT2A1 antibody (ab38416) stained human ES cell derived hepatocytes. The cells were fixed in methanol, then blocked and permeabilized using BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab38416 at a 1/250 dilution for 16 hours at 4oC. The secondary antibody was a Goat anti-Rabbit Alexa Fluor 568, used at a 1/400 dilution.

    See Abreview

Anti-SULT2A1 antibody (ab38416) 使用論文

This product has been referenced in:
  • Yalcin EB  et al. Downregulation of sulfotransferase expression and activity in diseased human livers. Drug Metab Dispos 41:1642-50 (2013). Read more (PubMed: 23775849) »
  • O'Shaughnessy PJ  et al. Steroidogenic enzyme expression in the human fetal liver and potential role in the endocrinology of pregnancy. Mol Hum Reprod : (2012). WB, IHC ; Human . Read more (PubMed: 23197595) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (ES - derived hepatocytes)
Specification ES - derived hepatocytes
Permeabilization Yes - During blocking step, BSA is diluted in PBS/0.1% Tween.
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Adreanl Carcinoma)
Specification Adreanl Carcinoma
Fixative Methanol
Permeabilization Yes - 1% TX100
Username

Abcam user community

Verified customer

投稿 Oct 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"