保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
バッファー Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
精製度 Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 98 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
機能 Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
組織特異性 Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
配列類似性 Belongs to the sulfatase family.
翻訳後修飾 The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
細胞内局在 Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
Information by UniProt
Extracellular sulfatase Sulf 2 antibody
Extracellular sulfatase Sulf-2 antibody
Anti-Sulfatase 2 antibody 画像
Western blot - Sulfatase 2 antibody (ab86368)
Anti-Sulfatase 2 antibody (ab86368) at 1.25 µg/ml + HepG2 cell lysate Secondary HRP conjugated anti-Rabbit IgG (1:50,000-100,000) Predicted band size : 98 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?) This antibody recognises a splice variant of Sulfatase 2.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sulfatase 2 antibody (ab86368)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Sulfatase 2 with ab86368 at 4-8ug/ml. Arrows indicate positively labelled epithelial cells of the renal tubule.
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