製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Sulfatase 2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human Sulfatase 2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 100 kDa).

ターゲット情報

  • 機能
    Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • 組織特異性
    Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
  • 配列類似性
    Belongs to the sulfatase family.
  • 翻訳後修飾
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 細胞内局在
    Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Extracellular sulfatase Sulf 2 antibody
    • Extracellular sulfatase Sulf-2 antibody
    • HSulf-2 antibody
    • HSulf2 antibody
    • Sulf2 antibody
    • SULF2_HUMAN antibody
    see all

画像

  • All lanes : Anti-Sulfatase 2 antibody (ab101057) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Sulfatase 2 contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed around 46 kDa is thought to be a splice variant of Sulfatase 2. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab101057 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101057 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab101057 has not yet been referenced specifically in any publications.

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