製品の概要

  • 製品名Anti-Sulfatase 2 antibody [2B4]
    Sulfatase 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B4] to Sulfatase 2
  • 特異性ab113405 shows no cross-reactivity with Sulfatase 1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant Human Sulfatase 2.

  • エピトープab113405 recognises an epitope located in the C terminal domain of Sulfatase 2.
  • ポジティブ・コントロール
    • Sulfatase 2 transfected HEK29 cells or non-small-cell Human lung carcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa. ab113405 detects a major band of approximately 50kDa in Sulfatase 2 transfected HEK293 cell lysates. A band at 135kDa (unprocessed protein), and a fragment at 37kDa, may also be present.

ターゲット情報

  • 機能Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.
  • 組織特異性Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.
  • 配列類似性Belongs to the sulfatase family.
  • 翻訳後修飾The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 細胞内局在Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Extracellular sulfatase Sulf 2 antibody
    • Extracellular sulfatase Sulf-2 antibody
    • HSulf-2 antibody
    • HSulf2 antibody
    • Sulf2 antibody
    • SULF2_HUMAN antibody
    see all

Anti-Sulfatase 2 antibody [2B4] 画像

  • Paraffin embedded HEK293 cells transfected with Sulfatase 2 stained with ab113405.

Anti-Sulfatase 2 antibody [2B4] (ab113405) 使用論文

This product has been referenced in:
  • Lemjabbar-Alaoui H  et al. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene 29:635-46 (2010). Read more (PubMed: 19855436) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK 293T Transfected with SULF2 over expressing pl)
Permeabilization Yes - .1% Triton x-100 in 1XPBS with 1 % Goat Serum
Specification HEK 293T Transfected with SULF2 over expressing pl
Blocking step 5% Goat serum in 1XPBS with 0.01% Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Jul 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"