製品の概要

  • 製品名Sucrose Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    > 0.2 µM
  • 検出範囲
    0 µM - 10 µM
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (?= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 保存方法Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Enzyme Mix (lyophilized) Green 1 vial
    Invertase (Lyophilized) Blue1 1 vial
    OxiRed Probe in DMSO Red 1 x 200µl
    Sucrose Assay Buffer WM 1 x 25ml
    Sucrose Standard (Lyophilised) Yellow 1 x 100µl
  • 研究分野
  • 関連性Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
  • 別名
    • Saccharose
    • Table sugar

アプリケーション

Our Abpromise guarantee covers the use of ab83387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Sucrose Assay Kit 画像

  • Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.

プロトコール

Sucrose Assay Kit (ab83387) 使用論文

ab83387 has not yet been referenced specifically in any publications.

Product Wall

The laboratory has reassured me that mannitol will not be detected. You will still want to include a negative control that leaves out the invertase, though, and subtract this value from the values of the samples that do receive invertase.

The laboratory confirmed that the buffer should be stable for six months from the date of shipment if stored and handled correctly.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"