Anti-Substance P 抗体 [SP-DE4-21] (ab14184)
製品の概要
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製品名Anti-Substance P antibody [SP-DE4-21]
Substance P 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [SP-DE4-21] to Substance P
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由来種Mouse
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特異性The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).
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アプリケーション適用あり: ELISA, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Guinea pig, Human
交差が予測される動物種: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
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免疫原
Synthetic peptide corresponding to Human Substance P aa 58-68.
Sequence:RPKPQQFFGLM
Database link: P20366 -
ポジティブ･コントロール
- Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: PBS, 0.1M Glycine. pH 7.4 -
Concentration information loading... -
精製度Protein G purified
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ポリ/モノモノクローナル
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クローン名SP-DE4-21
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab14184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/10000. | |
| IHC-P | 1/1000 - 1/2000. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
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配列類似性Belongs to the tachykinin family.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 281512 Cow
- Entrez Gene: 6863 Human
- Entrez Gene: 21333 Mouse
- Entrez Gene: 24806 Rat
- Omim: 162320 Human
- SwissProt: P19850 Chicken
- SwissProt: P01289 Cow
- SwissProt: P67932 Guinea pig
see all -
別名
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](http://a.abcam.com/ps/products/14/ab14184/Images/ab14184-240737-ab14184ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbsab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)](http://a.abcam.com/ps/products/14/ab14184/Images/Substance-P-Primary-antibodies-ab14184-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](http://a.abcam.com/ps/products/14/ab14184/Images/Substance-P-Primary-antibodies-ab14184-21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](http://a.abcam.com/ps/products/14/ab14184/Images/ab14184_1.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB. -
![ELISA - Anti-Substance P antibody [SP-DE4-21] (ab14184)](http://a.abcam.com/ps/products/14/ab14184/Images/Substance-P-Primary-antibodies-ab14184-33.jpg)
ELISA - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an anonymous Abreviewab14184 detecting Substance P in Human Serum samples by sandwich ELISA. Samples were blocked with 1% BSA for 1 hour at 25°C and incubated with the primary antibody (1/400 in PBS) for 20 hours at 4°C. A biotinylated Rabbit anti-Substance P polyclonal (1/200) was used as the secondary antibody.
参考文献
This product has been referenced in:
- Liang CC et al. Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats. Sci Rep 8:2105 (2018). IHC ; Rat . Read more (PubMed: 29391467) »
- Chou CM et al. Enhanced expressions of neurodegeneration-associated factors, UPS impairment, and excess Aß accumulation in the hippocampus of mice with persistent cerebral toxocariasis. Parasit Vectors 10:620 (2017). Read more (PubMed: 29273062) »
レビューと Q&A
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