製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to STXBP2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 310-524 of Human STXBP2 (AAH02869).

  • ポジティブ・コントロール
    • A431 and HepG2 cell lines. H1299 and HeLa whole cell lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
ICC/IF 1/200. Use with paraformaldehyde fixed cells.

ターゲット情報

  • 機能
    Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells.
  • 組織特異性
    Placenta, lung, liver, kidney and pancreas, as well as in peripheral blood lymphocytes.
  • 関連疾患
    Defects in STXBP2 are the cause of hemophagocytic lymphohistiocytosis familial type 5 (FHL5) [MIM:613101]. FHL5 is rare disorder characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, and less frequently neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits and ataxia.
  • 配列類似性
    Belongs to the STXBP/unc-18/SEC1 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FHL5 antibody
    • Hunc18b antibody
    • MUNC18 2 antibody
    • pp10122 antibody
    • Protein unc-18 homolog 2 antibody
    • Protein unc-18 homolog B antibody
    • STXB2_HUMAN antibody
    • Stxbp2 antibody
    • syntaxin binding protein 2 antibody
    • Syntaxin-binding protein 2 antibody
    • Unc-18B antibody
    • UNC18 2 antibody
    • Unc18-2 antibody
    • UNC18B antibody
    see all

画像

  • All lanes : Anti-STXBP2 antibody (ab103976) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 66 kDa
  • ab103976 (1/200) staining STXBP2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

参考文献

This product has been referenced in:
  • Vogel GF  et al. Disrupted apical exocytosis of cargo vesicles causes enteropathy in FHL5 patients with Munc18-2 mutations. JCI Insight 2:N/A (2017). Read more (PubMed: 28724787) »
  • Vogel GF  et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol 211:587-604 (2015). Read more (PubMed: 26553929) »

See all 2 Publications for this product

レビューと Q&A

Vielen Dank für Ihre Anfrage.

Leider analysieren wir routinemäßig keine Nebenbanden, die in unseren Blots auftauchen, da wir unsere Blots zb. auch nicht optimieren, und es sich daher um Artefakte handeln kann. Außerdem si...

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Thank you for contacting us.

Unfortunately ab103976 has never been tested in Flow Cytometry assays. It doesn’t mean it won’t work in this application, but we don’t have data to support it.

If you are interested in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Apr 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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