製品の概要

  • 製品名Anti-STX11 antibody
    STX11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STX11
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human STX11.

    (Peptide available as ab91441.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human brain tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab73375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 33 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network.
  • 関連疾患Defects in STX11 are the cause of hemophagocytic lymphohistiocytosis familial type 4 (FHL4) [MIM:603552]; also known as HPLH4. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • 配列類似性Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 細胞内局在Membrane. Golgi apparatus > trans-Golgi network membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FHL4 antibody
    • HLH4 antibody
    • HPLH4 antibody
    • Stx11 antibody
    • STX11_HUMAN antibody
    • Syntaxin 11 antibody
    • Syntaxin-11 antibody
    see all

Anti-STX11 antibody 画像

  • Anti-STX11 antibody (ab73375) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • IHC image of STX11 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73375, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab73375 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-STX11 antibody (ab73375) 使用論文

ab73375 has not yet been referenced specifically in any publications.

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