製品の概要

  • 製品名Anti-STUB1 antibody
    STUB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STUB1
  • 特異性Detects human carboxyl terminus of hsc70-interacting protein (CHIP).Detects a band of approximately 35 kDa representing CHIP from COS-1 cells overexpressing the human gene including a non-specific band at 80kDa . A customer reported that in mouse cerebrum the antibody detects a band of 35kDa as well as two small mon-specific at 44 and 70 kDa.
  • アプリケーション適用あり: WB, ICC/IF, Inhibition Assay, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide corresponding to Human STUB1 aa 218-232.
    Sequence:

    VDEKRKKRDIPDYLC


    (Peptide available as ab4934)

  • ポジティブ・コントロール
    • Mouse brain (please see references)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 35 kDa.Can be blocked with Human STUB1 peptide (ab4934). PubMed: 16207874(representing CHIP from COS-1 cells overexpressing the human gene). Block with 5% milk (see Abreview).
ICC/IF 1/50. PubMed: 16207874
Inhibition Assay 1/100.
IHC-P 1/100.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
  • 組織特異性Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 3 TPR repeats.
    Contains 1 U-box domain.
  • ドメインThe TPR domain is essential for ubiquitination mediated by UBE2D1.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen NY CO 7 antibody
    • Antigen NY-CO-7 antibody
    • C terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70 interacting protein antibody
    • Carboxy terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70p interacting protein antibody
    • CHIP antibody
    • CHIP_HUMAN antibody
    • CLL associated antigen KW 8 antibody
    • CLL-associated antigen KW-8 antibody
    • E3 ubiquitin protein ligase CHIP antibody
    • E3 ubiquitin-protein ligase CHIP antibody
    • Heat shock protein A binding protein 2 (c terminal) antibody
    • HSPABP2 antibody
    • NY CO 7 antibody
    • PP1131 antibody
    • SDCCAG7 antibody
    • Serologically defined colon cancer antigen 7 antibody
    • STIP1 homology and U Box containing protein 1 antibody
    • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
    • STIP1 homology and U box-containing protein 1 antibody
    • STUB 1 antibody
    • STUB1 antibody
    • UBOX 1 antibody
    • UBOX1 antibody
    see all

Anti-STUB1 antibody 画像

  • Western blot detection of transfected COS-1 cells expressing STUB1 using ab2917.
  • Western blot analysis of STUB1 was performed by loading 20ug of total protein extracted from the left hemisphere (Left lane)or right hemisphere (right lane) of a normal C57BL/6 mouse brain per well on an SDS-PAGE gel. Proteins were transferred to a membrane, blocked with 5% non-fat dry milkand probed with a STUB1 polyclonal antibody (ab2915) at a dilution of 1:1000, followed by a HRP-conjugated goat anti-rabbit IgG secondary antibody. Detection was performed using a chemiluminescent substrate.
  • ICC/IF image of ab2917 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2917, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

Anti-STUB1 antibody (ab2917) 使用論文

This product has been referenced in:
  • Shi C  et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS One 10:e0138936 (2015). Mouse . Read more (PubMed: 26414348) »
  • Sisoula C  et al. CHIP-dependent p53 regulation occurs specifically during cellular senescence. Free Radic Biol Med 50:157-65 (2011). WB, ICC/IF ; Human . Read more (PubMed: 20974249) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
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投稿 Jun 19 2006

Ab2917, lot 63868 has a concentration of 1 mg/ml. Have a nice day.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"