Anti-Stromal interaction molecule 1 抗体 (ab57834)

製品の概要

  • 製品名Anti-Stromal interaction molecule 1 antibody
    Stromal interaction molecule 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Stromal interaction molecule 1
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cyt, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 24-686 of Human Stromal interaction molecule 1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at 4 µg/mg of lysate.

ターゲット情報

  • 機能Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
  • 組織特異性Ubiquitously expressed in various human primary cells and tumor cell lines.
  • 関連疾患Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
  • 配列類似性Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • ドメインThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • 翻訳後修飾Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

Anti-Stromal interaction molecule 1 antibody 画像

  • Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.


  • Predicted band size : 77 kDa
    Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • ab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Stromal interaction molecule 1 antibody (ab57834) 使用論文

ab57834 has not yet been referenced specifically in any publications.

Product Wall

Any anti-mouse IgG secondary antibody will be a suitable secondary antibody. We have many choices of different conjugations. For an HRP-conjugated secondary, I suggest ab97023, and for a green fluorescent conjugate I suggest Alexa Fluor 488-conjugated ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for calling Abcam earlier today.

I am sorry about the issues you have been having with the current lot of ab57834. If you could provide me with either the Abcam order number or PO# as we discussed, I will be happy to send you a repl...

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Thank you for your reply.

These two antibodies are very similar. Ab57834 is supplied at a size of 100ug with a recommended working dilution in WB of 1:200-1:1000, while ab119288 is supplied at a size of 50ug with a recommended WB dilution of...

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Thank you for contacting us. The Stromal interaction molecule 1 antibody ab57834 was validated using 5% milk as a blocking agent. I hope this helps, please let me know if you need any additional information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Oct 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 1000 µg
Specification HeLa
Immuno-precipitation step Protein G
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Abcam user community

Verified customer

投稿 Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 40 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (HELA)
Total protein in input 200 µg
Specification HELA
Immuno-precipitation step Protein G
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Abcam user community

Verified customer

投稿 Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X
Username

Abcam user community

Verified customer

投稿 Aug 19 2010

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