Anti-Streptolysin O (Hemolytic streptococcus) 抗体 (ab188539)

製品の概要

  • 製品名
    Anti-Streptolysin O (Hemolytic streptococcus) antibody
  • 製品の詳細
    Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus)
  • 由来種
    Rabbit
  • 特異性
    ab188539 reacts with Streptolysin O of Group A, C and G origins.
  • アプリケーション
    適用あり: WB, Neutralising, IPmore details
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Streptolysin O (Hemolytic streptococcus). (from S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab188539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
Neutralising Use at an assay dependent concentration.

Neutralization of hemolysis by Streptolysin O (SLO).

IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
  • 別名
    • Streptolysin O (Thiol-activated cytolysin) slo antibody
    • Streptolysin O antibody

画像

  • All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

    Lane 1 : Medium only
    Lane 2 : Culture supernatant of GAS strain Sa
    Lane 3 : Culture supernatant of GCS strain H46A

    Predicted band size: 64 kDa



    Most of Streptolysin O in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of Streptolysin O in the culture of this strain. Condensed sample is analyzed in the second Western Blot.

     

    The two faint bands below Streptolysin O band are partially degraded Streptolysin O.

  • All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

    Lane 1 : Concentrated culture of GSA strain Sa
    Lane 2 : Concentrated medium only

    Predicted band size: 64 kDa



    Culture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation.

     

    The Streptolysin O of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B and both the concentration and size of Streptolysin O in culture medium is decreased from the native one.

参考文献

This product has been referenced in:
  • Hancz D  et al. Inhibition of Inflammasome-Dependent Interleukin 1ß Production by Streptococcal NAD(+)-Glycohydrolase: Evidence for Extracellular Activity. MBio 8:N/A (2017). Read more (PubMed: 28720729) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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