Streptavidin HRP (ready-to-use) (ab64269)
製品の概要
-
製品名
Streptavidin HRP (ready-to-use) -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.
This product was previously called Streptavidin-Peroxidase.
IHC protocol suitable for use with Streptavidin-HRP ab64269:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
製品の特性
-
製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 7.60
Preservative: 0.2% Proclin 300
Constituent: PBS -
Concentration information loading...
-
研究分野
-
関連性
Streptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64269の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (42)
ab64269 は 42 報の論文で使用されています。
- Luo L et al. Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells. Biotechnol J 17:e2100401 (2022). PubMed: 34921593
- Momin N et al. Maximizing response to intratumoral immunotherapy in mice by tuning local retention. Nat Commun 13:109 (2022). PubMed: 35013154
- Badr El-Din NK et al. Thymax, a gross thymic extract, exerts cell cycle arrest and apoptosis in Ehrlich ascites carcinoma in vivo. Heliyon 8:e09047 (2022). PubMed: 35299600
- Hires M et al. Glycan signatures for the identification of cisplatin-resistant testicular cancer cell lines: Specific glycoprofiling of human chorionic gonadotropin (hCG). Cancer Med 11:968-982 (2022). PubMed: 35044085
- Matouskova K et al. Exposure to Low Doses of Oxybenzone During Perinatal Development Alters Mammary Gland Stroma in Female Mice. Front Toxicol 4:910230 (2022). PubMed: 35669359