The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
anti-Strep-Tag II antibody
Strep-Tag II antibody
Strep-Tag II antibody antibody
Strep-Tag IIantiboy antibody
Western blot - Streptavidin antibody (ab76950)
All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml
Lane 1 : Streptavidin tagged recombinant protein with blocking peptide Lane 2 : Streptavidin tagged recombinant protein