製品の概要

  • 製品名
    Anti-Strep-tag II antibody
    Strep-tag II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Strep-tag II
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    tag
  • 免疫原

    Amino acid sequence

    ASWSHPQFEKGA

  • ポジティブ・コントロール
    • Streptavidin tagged recombinant protein
  • 特記事項


    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity chromatography purified via peptide column
  • 一次抗体 備考
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml.

Predicted Band Size: 24kDa

ターゲット情報

  • 関連性
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • 別名
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Anti-Strep-tag II antibody 画像

  • All lanes : Anti-Strep-tag II antibody (ab76949) at 1 µg/ml

    Lane 1 : StreptII tagged recombinant protein at 0.005 µg
    Lane 2 : StrepII tagged recombinant protein at 0.025 µg

プロトコール

Anti-Strep-tag II antibody (ab76949) 使用論文

This product has been referenced in:
  • McCourt JL  et al. In vitro stability of therapeutically relevant, internally truncated dystrophins. Skelet Muscle 5:13 (2015). WB ; Fall armyworm . Read more (PubMed: 25954502) »
  • Allen CE  et al. Analysis of novel iron-regulated, surface-anchored hemin-binding proteins in Corynebacterium diphtheriae. J Bacteriol 195:2852-63 (2013). Read more (PubMed: 23585541) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Jurkat T cells
Blocking step
Western Blotting Reagent (Roche) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Sep 20 2017

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Chicken Cell lysate - whole cell (Lymphocytes)
Specification
Lymphocytes
Total protein in input
100 µg
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投稿 Jan 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Hamster Cell lysate - whole cell (CHO whole cell lysate)
Specification
CHO whole cell lysate
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 23 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (AD293)
Specification
AD293
Treatment
Strep-tagged keratin
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (Strep-tagged recombinant protein)
Loading amount
0.5 µg
Specification
Strep-tagged recombinant protein
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (cell lysate containing expressed recombinant prote)
Loading amount
25 µg
Specification
cell lysate containing expressed recombinant prote
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Dec 03 2012

Vielen Dank für Ihre Anfrage.

Wir haben verschiedene Antikörper die ich Ihnen in diesem Fall empfehlen kann. Die drei Produkte unterscheiden sich in der Hostspezies.

1. Chicken polyclonal Secondary Antibody to Rabbit IgG - H...

Read More

Thank you for your reply.

I have processed your request to receive ab119810 as free of charge replacement for ab76949. Currently, ab119810 is out of stock, but we expect to be able to ship it to you by 11th May, I do apologize for this delay....

Read More

Thank you for your reply.

Both antibodies should work equally as well for you. I was suggested ab119810, as it is the only other Strep tag antibody we have in stock and the only information that we have on it, is what is already on the webpag...

Read More

Thank you for your reply.

I think the best way forward is too either replace or refund the cost of ab76949. An alternative to ab76949 is ab119810:

http://www.abcam.com/Strep-tag-II-antibody-ab119810.html

Please let me know...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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