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Synthetic peptide derived from within residues 1 - 100 of Mouse Stra8.
(Peptide available as ab49601.)
Our Abpromise guarantee covers the use of ab49602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Ab49602 was tested on a dual-tagged Stra8 (Mouse) overexpression lysate predicted to run at 60 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18799790|
ab49602 at 1/250 dilution, staining Stra8 in Primordial Germ Cells from 13.5 days post-coitum mouse ovaries and testis by Immunocytochemistry/ immunofluorescence. The cells were fixed by 4% paraformaldehyde in PBS for 10 min at room temperature and then permeabilized for 10 min in 0.1% Triton X-100 in PBS. After a 1-h block in 5% bovine serum albumin in PBS, primary antibody was added, diluted in 0.5% BSA in PBS and incubated with sample overnight at 4°C. An Alexa Fluor®568 conjugated Goat anti-rabbit IgG was used as secondary for 1 hour. Nuclei were labeled with Hoechst 33349 (1µg/ml). A rabbit IgG was also used as control antibody with the same procedure.
ab49602 staining Stra8 (red) in hamster fetal ovary tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS + 0.2% Triton X-100 + 10% donkey serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (10µg/ml) was used as the secondary antibody. Nuclei stained blue with DAPI.
Frozen mouse testis tissue stained for Stra8 using ab49602 at 1/3000 dilution in immunohistochemical analysis. Phalloidin (red), which labels F-actin was used as a counterstain.
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