Anti-STON2 抗体 (ab121165)
Key features and details
- Rabbit polyclonal to STON2
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-STON2 antibody -
製品の詳細
Rabbit polyclonal to STON2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 299-437 of Human STON2.
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ポジティブ・コントロール
- IHC-P: Human fallopian tube tissue; ICC: A431 cells; WB: K562 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 101 kDa.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 101 kDa. |
ターゲット情報
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機能
Adapter protein involved in endocytic machinery. May be involved in the vesicle recycling. May facilitate clathrin-coated vesicle uncoating. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the Stoned B family.
Contains 1 MHD (mu homology) domain.
Contains 1 SHD (stonin homology) domain. -
ドメイン
The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of SYT1, SYT2, EPS15, EPS15R and ITSN1. -
細胞内局在
Cytoplasm. Membrane. Some fraction is membrane-associated. - Information by UniProt
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参照データベース
- Entrez Gene: 85439 Human
- Omim: 608467 Human
- SwissProt: Q8WXE9 Human
- Unigene: 14248 Human
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別名
- 4933401N24Rik antibody
- AI425999 antibody
- AW544599 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling STON2 with ab121165 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab121165 at 4 µg/ml staining STON2 in PFA/Triton X-100 fixed A431 cells by Immunofluorescence(green).
Note: positivity in nucleoli and cytoplasm. -
Anti-STON2 antibody (ab121165) + Human cell line K562
Predicted band size: 101 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling STON2 with ab121165 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling STON2 with ab121165 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling STON2 with ab121165 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121165 は論文での使用が確認できていません。