STO whole cell lysate (ab3874)

製品の概要

  • 製品名STO whole cell lysate
  • 特記事項Source - Mouse embryonic fibroblasts.


    STO whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーConstituent: 5% Beta mercaptoethanol
    Note: 1xSDS buffer
  • Concentration information loading...
  • 精製度Whole Cell Lysate
  • ライゼート 備考STO whole cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF,5 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 2 µg/ml Leupeptin, 1 mM Na3VO4, 1 mM NaF). Debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野
  • 背景These immortal cells are used as a monolayer of feeder cells to embryonic stem cells (in particular in human stem cell research). Cells are SIM mouse embryo-derived and thioguanine and ouabain resistant.

アプリケーション

Our Abpromise guarantee covers the use of ab3874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. STO whole cell lysate is ready to load on SDS-PAGE for Western blotting. We recommend loading 20 µg per lane for mini gels.

STO whole cell lysate (ab3874) 使用論文

ab3874 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam and for your interest in our whole cell lysates.

Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
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Thank you very much for contacting us with your enquiry.

I'm sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell ...

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