The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
1/50 - 1/100.
Expressed in testis.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Nucleus. Nucleus > nucleolus. Cytoplasm. When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.
ab37974, at a 1/50 dilution, staining STK35 in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37974 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.