Anti-STK31 抗体 - N-terminal (ab71698)

製品の概要

  • 製品名
    Anti-STK31 antibody - N-terminal
    STK31 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STK31 - N-terminal
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide from the N terminal region of human STK31 conjugated to KLH.

  • ポジティブ・コントロール
    • Rat testis tissue lysate. Human breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab71698 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 116 kDa).

ターゲット情報

  • 組織特異性
    Testis specific.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Testis tissue sperm-binding protein Li 78m antibody
    • Tudor domain containing 8 antibody
    see all

画像

  • Anti-STK31 antibody - N-terminal (ab71698) at 1/100 dilution + rat testis tissue lysate at 12.5 µg

    Predicted band size : 116 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab71698, at 1/50 dilution, staining STK31 in human breast carcinoma by Immunohistochemistry using formalin fixed, paraffin embedded tissue. ab71698 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

参考文献

ab71698 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Loading amount
20 µg
Specification
ES cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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