Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Protein G purified
ab71698 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 116 kDa).
Anti-STK31 antibody - N-terminal 画像
Western blot - STK31 antibody - N-terminal (ab71698)
Anti-STK31 antibody - N-terminal (ab71698) at 1/100 dilution + rat testis tissue lysate at 12.5 µg
Predicted band size :
Observed band size :
100 kDa (
why is the actual band size different from the predicted?
Additional bands at :
32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STK31 antibody - N-terminal (ab71698)
ab71698, at 1/50 dilution, staining STK31 in human breast carcinoma by Immunohistochemistry using formalin fixed, paraffin embedded tissue. ab71698 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
Anti-STK31 antibody - N-terminal (ab71698) 使用論文
has not yet been referenced specifically in any publications.
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