Anti-STK3 (phospho T180) + MST1 (phospho T183) 抗体 (ab79199)

製品の概要

  • 製品名
    Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody
  • 製品の詳細
    Rabbit polyclonal to STK3 (phospho T180) + MST1 (phospho T183)
  • 特異性
    ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3).
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).

  • ポジティブ・コントロール
    • WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

ターゲット情報

Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody 画像

  • All lanes : Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody (ab79199)

    Lane 1 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins)
    Lane 2 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins) with immunising phosphopeptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody (ab79199) 使用論文

This product has been referenced in:
  • Wu W  et al. Early protective role of MST1 knockdown in response to experimental diabetic nephropathy. Am J Transl Res 8:1397-411 (2016). Read more (PubMed: 27186267) »
  • Dixit D  et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »

See all 3 Publications for this product

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Since ab79199 has not been tried on mouse samples, we do not guarantee it for this species. The guarantee applies for tested species and applications only.

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I am sorry to hear that the antibodies are not working as expected.

Your protocol looks fine to me. I wanted to ask you a few more questions:

1) Both antibodies detect a phospho-site. Under the expe...

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