製品の概要

  • 製品名Anti-STIP1 antibody
    STIP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STIP1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: QRCMMAQYNR HDSPEDVKRR AMADPEVQQI MSDPAMRLIL EQMQKDPQAL, corresponding to a region within C terminal amino acids 469-518 of Human STIP1

  • ポジティブ・コントロール
    • Human muscle. Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

Anti-STIP1 antibody 画像

  • Anti-STIP1 antibody (ab65046) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling STIP1 with ab65046 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-STIP1 antibody (ab65046) 使用論文

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"