製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to STIM2
  • 特異性
    ab59342 is predicted to have no cross-reactivity to STIM1.
  • アプリケーション
    適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    An 18 amino acid peptide from near the carboxy terminus of human STIM2.

  • ポジティブ・コントロール
    • A20 cell lysate. IF: HeLa cell line

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 84 kDa).Can be blocked with STIM2 peptide (ab176417).
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • 組織特異性
    Expressed in all tissues and tumor cell lines examined.
  • 配列類似性
    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻訳後修飾
    Glycosylated.
    Phosphorylated predominantly on Ser residues.
  • 細胞内局在
    Endoplasmic reticulum membrane. Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ39527 antibody
    • KIAA1482 antibody
    • STIM 2 antibody
    • STIM2 antibody
    • STIM2_HUMAN antibody
    • Stromal interaction molecule 2 antibody
    see all

画像

  • Lane 1 : Anti-STIM2 antibody (ab59342) at 0.5 µg/ml
    Lane 2 : Anti-STIM2 antibody (ab59342) at 1 µg/ml
    Lane 3 : Anti-STIM2 antibody (ab59342) at 2 µg/ml

    Lane 1 : A-20 cell lysate
    Lane 2 : A-20 cell lysate
    Lane 3 : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 84 kDa
  • ICC/IF image of ab59342 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59342, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Bréchard S  et al. STIM1 but not STIM2 is an essential regulator of Ca2+ influx-mediated NADPH oxidase activity in neutrophil-like HL-60 cells. Biochem Pharmacol 78:504-13 (2009). Read more (PubMed: 19433064) »
  • Chiu TY  et al. Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead ions. Toxicol Sci 110:353-62 (2009). Read more (PubMed: 19429662) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (microsomal fraction)
Loading amount
30 µg
Specification
microsomal fraction
Gel Running Conditions
Reduced Denaturing (bis-tris 4-20% gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Apr 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (microsomal fraction)
Loading amount
20 µg
Specification
microsomal fraction
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Sep 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (affinity purified)
Specification
affinity purified
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type
Direct
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投稿 Sep 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse skeletal muscle)
Loading amount
30 µg
Specification
mouse skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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投稿 Aug 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells with transfected human stim2)
Loading amount
20 µg
Specification
293 cells with transfected human stim2
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Aug 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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