Anti-Steroidogenic Factor 1 抗体 (ab103679)

製品の概要

  • 製品名Anti-Steroidogenic Factor 1 antibody
    Steroidogenic Factor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Steroidogenic Factor 1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 70-100 of Human Steroidogenic Factor 1.

  • ポジティブ・コントロール
    • HepG2 cell lysate; Human spleen tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3.
  • 関連疾患Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
    Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
    Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻訳後修飾Acetylation stimulates the transcriptional activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AD4BP antibody
    • Adrenal 4 binding protein antibody
    • Adrenal 4-binding protein antibody
    • ELP antibody
    • FTZ 1 antibody
    • FTZ1 antibody
    • FTZF 1 antibody
    • FTZF1 antibody
    • Fushi tarazu factor (Drosophila) homolog 1 antibody
    • Fushi tarazu factor homolog 1 antibody
    • NR5A1 antibody
    • Nuclear receptor AdBP4 antibody
    • Nuclear receptor subfamily 5 group A member 1 antibody
    • POF7 antibody
    • SF 1 antibody
    • SF-1 antibody
    • SF1 antibody
    • SPGF8 antibody
    • SRXY3 antibody
    • Steroid hormone receptor Ad4BP antibody
    • Steroidogenic factor 1 antibody
    • Steroidogenic factor 1 nuclear receptor antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    • STF1_HUMAN antibody
    see all

Anti-Steroidogenic Factor 1 antibody 画像

  • Anti-Steroidogenic Factor 1 antibody (ab103679) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 52 kDa
  • ab103679, at 1/50 diltuion, staining Steroidogenic Factor 1 in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

Anti-Steroidogenic Factor 1 antibody (ab103679) 使用論文

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Aug 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Ovary)
Specification Ovary
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2012

Thank you for your message and for providing this further information.

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