Anti-Steroid sulfatase 抗体 (ab83668)

Submit an Abreview Q&A (1)

製品の概要

  • 製品名
    Anti-Steroid sulfatase antibody
    Steroid sulfatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Steroid sulfatase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (EPTSNMDIFP TVAKLAGAPL PEDRIIDGRD LMPLLEGKSQ RSDHEFLFHY) of Human Steroid sulfatase (NP_000342).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Human Placenta lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Steroid sulfatase peptide (ab199997). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Conversion of sulfated steroid precursors to estrogens during pregnancy.
  • 関連疾患
    Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.
  • 配列類似性
    Belongs to the sulfatase family.
  • 翻訳後修飾
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ARSC antibody
      • ARSC1 antibody
      • Arylsulfatase C antibody
      • Arylsulfatase C isozyme S antibody
      • ASC antibody
      • EC 3.1.6.2 antibody
      • ES antibody
      • Estrone sulfatase antibody
      • SSDD antibody
      • Steroid sulfatase (microsomal) antibody
      • Steroid sulfatase (microsomal) arylsulfatase C isozyme S antibody
      • Steroid sulfatase antibody
      • Steryl sulfatase antibody
      • Steryl sulfatase precursor antibody
      • Steryl sulfate sulfohydrolase antibody
      • Steryl-sulfatase antibody
      • Steryl-sulfate sulfohydrolase antibody
      • STS antibody
      • STS_HUMAN antibody
      see all

    画像

    • Western blot - Steroid sulfatase antibody (ab83668)
      Western blot - Steroid sulfatase antibody (ab83668)
      Anti-Steroid sulfatase antibody (ab83668) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 63 kDa
      Observed band size: 63 kDa



      Gel concentration: 12%

    プロトコール

    参考文献

    ab83668 has not yet been referenced specifically in any publications.

    ab83668 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    do you also sell the peptide which was used to immunize animals to produce this antibody.

    I have been in contact with the lab and they have agreed to add this product to the catalogue.

    The peptide for ab83668 has been added to the catalogue under reference number ab199997, Steroid sulfatase peptide.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録