製品の概要

  • 製品名Anti-Stella antibody
    Stella 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Stella
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Mouse Stella.

    (Peptide available as ab23324.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal for Western Blot in Mouse Embryonic Germ Cell, Embryonic Stem Cell and Embryonic Carcinoma Cell Lysates. This antibody gave a positive signal for Immunofluorescence in Mouse Embryonic Stem cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 4 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能May play a role in maintaining cell pluripotentiality.
  • 組織特異性Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • 発生段階Expressed in fetal ovary.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
  • 別名
    • Compaction associated protein 1 antibody
    • Developmental pluripotency associated 3 antibody
    • Developmental pluripotency associated protein 3 antibody
    • Developmental pluripotency-associated protein 3 antibody
    • Dppa3 antibody
    • DPPA3_HUMAN antibody
    • PGC7 antibody
    • Stella related protein antibody
    • Stella-related protein antibody
    see all

Anti-Stella antibody 画像

  • The image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.

  • All lanes : Anti-Stella antibody (ab19878) at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml
    Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml
    Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence analysis of porcine induced pluripotent stem cells cultured in 2i + LIF medium, staining Stella with ab19878.

    Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked in 5% BSA. Cells were incubated with primary antibody (1/60) overnight at 4°C.

Anti-Stella antibody (ab19878) 使用論文

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Roper SJ  et al. ADP-ribosyltransferases Parp1 and Parp7 safeguard pluripotency of ES cells. Nucleic Acids Res 42:8914-27 (2014). ICC/IF ; Mouse . Read more (PubMed: 25034692) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, embryonic day 15.5
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Apr 06 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Loading amount 200000 cells
Specification mouse embryonic stem cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

投稿 Oct 24 2012

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 16°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2012

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided; the protocol looks fine to me to however I have following further suggestions for impro...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult ovary)
Specification Adult ovary
Fixative Formaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

投稿 Aug 29 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Marmoset adult testis)
Specification Marmoset adult testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target retrieval solution by Dako
Permeabilization Yes - Triton-X
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

煜欽 林

Verified customer

投稿 Aug 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embyro)
Specification Embyro
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Username

Abcam user community

Verified customer

投稿 Nov 14 2006

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"