Anti-Stathmin 1 (phospho S24) 抗体 [EP2124Y] (ab62336)

製品の概要

  • 製品名Anti-Stathmin 1 (phospho S24) antibody [EP2124Y]
    Stathmin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2124Y] to Stathmin 1 (phospho S24)
  • 特異性This antibody detects Stathmin 1 phosphorylated at Serine 24.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Phospho specific peptide corresponding to residues surrounding Serine 24 of human Stathmin 1.

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
    • 組織特異性Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
    • 配列類似性Belongs to the stathmin family.
      Contains 1 SLD (stathmin-like) domain.
    • 翻訳後修飾Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C1orf215 antibody
      • Lag antibody
      • LAP 18 antibody
      • LAP18 antibody
      • Leukemia associated phosphoprotein p18 antibody
      • Leukemia-associated phosphoprotein p18 antibody
      • Metablastin antibody
      • Oncoprotein 18 antibody
      • OP 18 antibody
      • Op18 antibody
      • p18 antibody
      • p19 antibody
      • Phosphoprotein 19 antibody
      • Phosphoprotein p19 antibody
      • pp17 antibody
      • pp19 antibody
      • PR22 antibody
      • Pr22 protein antibody
      • Prosolin antibody
      • Protein Pr22 antibody
      • SMN antibody
      • Stathmin antibody
      • Stathmin1 antibody
      • STMN 1 antibody
      • Stmn1 antibody
      • STMN1_HUMAN antibody
      see all

    Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] 画像

    • All lanes : Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336) at 1/1000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS+Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336) 使用論文

    This product has been referenced in:
    • Zhao E  et al. Stathmin mediates hepatocyte resistance to death from oxidative stress by down regulating JNK. PLoS One 9:e109750 (2014). WB . Read more (PubMed: 25285524) »

    See 1 Publication for this product

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    To confirm:

    1. Growing cells were starved for 24hr in the medium without FBS.

    2. Growing cells treated the with 20% FBS for 3hr.

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