Mouse monoclonal [STAAD23A] to STAT6
HeLa nuclear extract.
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Protein G purified
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/100. Predicted molecular weight: 94 kDa.
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Information by UniProt
IL 4 STAT antibody
Western blot - STAT6 antibody [STAAD23A] (ab51022)
Anti-STAT6 antibody [STAAD23A] (ab51022) at 1/100 dilution + HT1080 whole cell lysate at 25 µg
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique
Predicted band size :
Observed band size :
100 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry - STAT6 antibody [STAAD23A] (ab51022)
HeLa cells stained with ab51022 at 1/10 dilution, followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200 dilution.
Flow Cytometry - STAT6 antibody [STAAD23A] (ab51022)
HeLa cells were labelled with ab51022 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/400 dilution.
has not yet been referenced specifically in any publications.
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