製品の概要
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製品名Anti-STAT6 antibody [E265]
STAT6 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [E265] to STAT6
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由来種Rabbit
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特異性ab32108 recognises Stat-6. It does not cross react with other STAT family members
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アプリケーション適用あり: WB, Flow Cyt, IP, ICC/IFmore details
適用なし: IHC -
種交差性交差種: Human
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免疫原
Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- HeLa cells
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名E265
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab32108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa). | |
| Flow Cyt | 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/30. | |
| ICC/IF | 1/250 - 1/500. |
ターゲット情報
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機能Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
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配列類似性Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
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細胞内局在Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Information by UniProt
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参照データベース
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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別名
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
画像
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![Western blot - Anti-STAT6 antibody [E265] (ab32108)](http://a.abcam.com/ps/products/32/ab32108/Images/ab32108-298743-ab32108-KO-WB-EWAR.jpg)
Western blot - Anti-STAT6 antibody [E265] (ab32108)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [E265] (ab32108)](http://a.abcam.com/ps/products/32/ab32108/Images/ab32108_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [E265] (ab32108)Immunofluorescent staining of HeLa cells using ab32108 -
![Western blot - Anti-STAT6 antibody [E265] (ab32108)](http://a.abcam.com/ps/products/32/ab32108/Images/ab32108_1.jpg)
Western blot - Anti-STAT6 antibody [E265] (ab32108)Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
Predicted band size: 94 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?)
参考文献
ab32108 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"