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RabMAb

Anti-STAT6 抗体 [E265] (ab32108)

製品の概要

  • 製品名
    Anti-STAT6 antibody [E265]
    STAT6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E265] to STAT6
  • 由来種
    Rabbit
  • 特異性
    ab32108 recognises Stat-6. It does not cross react with other STAT family members
  • アプリケーション
    適用あり: WB, Flow Cyt, IP, ICC/IFmore details
    適用なし: IHC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HeLa cells
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/30.
ICC/IF 1/250 - 1/500.
  • 追加情報
    Is unsuitable for IHC.
  • ターゲット情報

    • 機能
      Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
    • 配列類似性
      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • 翻訳後修飾
      Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
    • 細胞内局在
      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent staining of HeLa cells using ab32108
    • Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
      Predicted band size: 94 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab32108 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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