製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to STAT6
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, ICC, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human STAT6 (C terminal).

  • ポジティブ・コントロール
    • Jurkat cells. Tonsil or Placenta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab44718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-Fr Use at an assay dependent concentration. See Abreview.
ICC Use at an assay dependent concentration. See Abreview.
WB 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
IP 1/400.
IHC-P 1/400.

ターゲット情報

  • 機能
    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • 配列類似性
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾
    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 antibody 画像

  • ab44718 staining STAT6 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

  • ab44718 staining STAT6 in human AGS gastric carcinoma cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X in TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (green) used at a 1/500 dilution.

    See Abreview

Anti-STAT6 antibody (ab44718) 使用論文

This product has been referenced in:
  • Chen J  et al. Aggravated myocardial infarction-induced cardiac remodeling and heart failure in histamine-deficient mice. Sci Rep 7:44007 (2017). WB . Read more (PubMed: 28272448) »
  • Malek Mohammadi M  et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med 9:265-279 (2017). Read more (PubMed: 28053183) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry.

Any references we know of which have used ab44718 will be noted under the 'Specific references' tab on our online datasheet for this product. At this time we do not know of any publications using ab44718.

Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification
NIH3T3 Mouse Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

投稿 Jan 12 2011

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain sections)
Specification
Mouse brain sections
Username

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投稿 Jan 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input
200 µg
Specification
NIH3T3 Mouse Fibroblasts
Immuno-precipitation step
Protein A/G
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Verified customer

投稿 Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 Mouse Fibroblasts)
Loading amount
10 µg
Specification
NIH 3T3 Mouse Fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

投稿 Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Total protein in input
200 µg
Specification
AGS Gastric Carcinoma cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

投稿 May 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount
20 µg
Specification
AGS Gastric Carcinoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Gastric Carcinoma cells)
Specification
AGS Gastric Carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Apr 29 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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