Anti-STAT5a (phospho Y694) 抗体 [6.F1] (ab106095)

製品の概要

  • 製品名
    Anti-STAT5a (phospho Y694) antibody [6.F1]
    STAT5a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
  • 特異性
    ab106095 is specific for STAT5a protein phosphorylated at Y694.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Y694 of Mouse STAT5a protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    NK92 cell lysate; Human breast tissue; 3T3 cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    6.F1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 91 kDa.
IHC-P Use a concentration of 20 - 40 µg/ml.
ICC/IF 1/50 - 1/1000.

ターゲット情報

  • 機能
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • 配列類似性
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Anti-STAT5a (phospho Y694) antibody [6.F1] 画像

  • All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)

    Lane 1 : NK92 cell lysate, untreated
    Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)

    Secondary
    Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution

    Predicted band size : 91 kDa
  • ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
  • ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.

Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) 使用論文

ab106095 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6 - using a pressure cooker
Permeabilization
Yes - Triton100-X
Specification
Mammary gland
Blocking step
MOM Mouse IgG blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Username

Flavia Ghiraldini

Verified customer

投稿 Sep 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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