The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.1 µg/ml.
1/100. Staining of formalin fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 90 kDa.
機能Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
配列類似性Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
翻訳後修飾Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
細胞内局在Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.