Anti-STAT5 抗体 [9F7] (ab230670)
Key features and details
- Mouse monoclonal [9F7] to STAT5
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-STAT5 antibody [9F7]
STAT5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9F7] to STAT5 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human STAT5.
Database link: P42229 -
ポジティブ・コントロール
- WB: STAT5-transfected HEK-293T cell lysate; HepG2, HeLa, SVT2, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts. IHC-P: HUman testis tissue. ICC/IF: COS7 cells. Flow Cyt (Intra): HeLa and Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
9F7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab230670の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
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特記事項 |
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WB
1/500 - 1/2000. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. |
ターゲット情報
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機能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Entrez Gene: 24918 Rat
- Entrez Gene: 25126 Rat
- Omim: 601511 Human
- Omim: 604260 Human
see all -
別名
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
画像
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All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution
Lane 1 : Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : STAT5-transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique. -
All lanes : Anti-STAT5 antibody [9F7] (ab230670) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique. -
Formalin-fixed, paraffin-embedded human testis tissue stained for STAT5 with ab230670 at 10 μg/ml in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with STAT5 and stained for STAT5 (green) using ab230670 at 1/100 dilution.
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Intracellular flow cytometric analysis ofHeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).
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Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling STAT% with ab230670 at 1/100 dilution (red) compared with a negative control (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab230670 は 7 報の論文で使用されています。
- Zhang X et al. Aging affects the biological activity of fibroblast growth factor (FGF) in gastric epithelial cell, which is partially rescued by uridine. Bioengineered 13:3724-3738 (2022). PubMed: 35105283
- Zhang B et al. Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization via the ECM1/STAT5 Pathway. Front Med (Lausanne) 8:796743 (2021). PubMed: 35141243
- Wu CH et al. Increased Expression of Thymic Stromal Lymphopoietin in Chronic Constriction Injury of Rat Nerve. Int J Mol Sci 22:N/A (2021). PubMed: 34281158
- Wang R & Huang K CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL-2 and TGF-ß by CD4+ T cells via the STAT5 signaling pathway. Mol Med Rep 21:2522-2532 (2020). PubMed: 32323817
- Wang L et al. Anti-CCL22 increases regulatory T cells in CD4+ T cells of rheumatoid arthritis patients via STAT5 pathway. Exp Ther Med 19:2127-2132 (2020). PubMed: 32104275
- Dong Z et al. CCL26 regulates the proportion of CD4+CD25+FOXP3+ Tregs and the production of inflammatory factors in peripheral blood mononuclear cells following acute ischemic stroke via the STAT5 pathway. Exp Ther Med 20:3343-3351 (2020). PubMed: 32855707
- Li X et al. Rat bone mesenchymal stem cells exert antiproliferative effects on nicotine-exposed T cells via iNOS production. Mol Med Rep 21:2267-2275 (2020). PubMed: 32186760