Anti-STAT4 抗体 [EP1900Y] (ab68156)

製品の概要

  • 製品名Anti-STAT4 antibody [EP1900Y]
    STAT4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1900Y] to STAT4
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human STAT4 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14765

  • ポジティブ・コントロール
    • WB: Daudi, K562 and Hela cell lysates. IHC-P: Human tonsil tissue sections.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000. Detects a band of approximately 30, 86 kDa (predicted molecular weight: 86 kDa).
IP 1/20.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • 関連疾患Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • 配列類似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • 細胞内局在Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Anti-STAT4 antibody [EP1900Y] 画像

  • All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry

Anti-STAT4 antibody [EP1900Y] (ab68156) 使用論文

ab68156 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human Vgamma9 Vdelta2 T cells)
Total protein in input 3e+006 cells
Immuno-precipitation step Other - Dynabeads (Protein G)
Specification human Vgamma9 Vdelta2 T cells
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投稿 Nov 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Purified human Vgamma9 Vdelta2 T-cells)
Permeabilization Yes - Foxp3 Staining Buffer Set
Gating Strategy CD3 and V delta 2 positive population
Specification Purified human Vgamma9 Vdelta2 T-cells
Fixation Foxp3 Staining Buffer Set
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投稿 Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"