Anti-STAT3 (phospho Y705) 抗体 [EP2147Y] (ab76315)

製品の概要

  • 製品名Anti-STAT3 (phospho Y705) antibody [EP2147Y]
    STAT3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2147Y] to STAT3 (phospho Y705)
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STAT3 aa 650-750 (phospho Y705).
    Database link: P40763
    (Peptide available as ab179551)

  • ポジティブ・コントロール
    • WB: HeLa cell lysate treated with alpha-interferon. IHC-P: Human colon adenocarcinoma and kidney tissues. IP: A431 cells treated with EGF.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200000. Predicted molecular weight: 88 kDa.
IP 1/20.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/500.

ターゲット情報

  • 機能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 関連疾患Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 配列類似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 参照データベース
  • 別名
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Anti-STAT3 (phospho Y705) antibody [EP2147Y] 画像

  • All lanes : Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) at 1/200000 dilution (purified)

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with IFN-a

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling STAT3 (phospho Y705) with purified ab76315 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa +/- IFN-α (50ng/mL, 5 minutes) cells labelling STAT3 (phospho Y705) with ab76315 at 1/500 (4.3 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.

  • Dot blot analysis of STAT3 single phospho peptide pY705 (lane 1) and STAT3 non-phospho peptide (lane 2) with ab76315 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling STAT3 (phospho Y705) with unpurified ab76315 at 1/100.

  • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue using  untreated (left) or alkaline phosphatase-treated (right) labeling STAT3 (phospho Y705) with ab76315 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H& L (HRP) (ab97051) at 1/500 dilution.

    Counter stained with Hematoxylin.

  • ab76315 (purified) at 1/30 immunoprecipitating STAT3 (phospho Y705) in A431 cell lysate treated with EGF. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-STAT3 (phospho Y705) antibody [EP2147Y] (ab76315) 使用論文

This product has been referenced in:
  • Peng HY  et al. MPT0B098, a Microtubule Inhibitor, Suppresses JAK2/STAT3 Signaling Pathway through Modulation of SOCS3 Stability in Oral Squamous Cell Carcinoma. PLoS One 11:e0158440 (2016). WB ; Human . Read more (PubMed: 27367272) »
  • Mishra V  et al. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ 7:16 (2016). Mouse . Read more (PubMed: 26949510) »

See all 27 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (peripheral blood CD19+ B cells)
Loading amount 200 µg
Specification peripheral blood CD19+ B cells
Treatment (1) Untreated (2) 100 ng recombinant hIL-10 for 24hours
Gel Running Conditions Reduced Denaturing (10 % SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Jul 07 2010

Application ELISA
Sample Human Cell (HEK293 cells stably overexpressing the human Lepti)
Specification HEK293 cells stably overexpressing the human Lepti
Blocking step proprietary blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Type Other
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Verified customer

投稿 Sep 12 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Lung)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 22 µg
Specification Lung
Blocking step Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Pete Serbedzija

Verified customer

投稿 Jul 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human esophageal cancer subcutaneous xenograft gro)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Specification human esophageal cancer subcutaneous xenograft gro
Blocking step Non-Abcam blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde
Username

Ms. Tatiana Karakasheva

Verified customer

投稿 Feb 22 2016

Application Western blot
Sample Human Cell lysate - whole cell (human normal oesopheal cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment 10 nM PMA
Specification human normal oesopheal cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Aug 27 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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投稿 Oct 29 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4–15%)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jul 30 2014

Thank you for your enquiry.

I can confirm that for the western blot image on the datasheet for ab76315, the HeLa cellswere serum-starved overnight, and then treated with 1000 U/ml IFN alpha for 15min at 37 degrees C.

This may requ...

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Thank you for contacting us. Please be advised that the ab76315 is not a purified antibody; it is in tissue culture supernatant which may contains other proteins also. We generally do not determine the antibody concentration that is prepared as tissue ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"