製品の概要

  • 製品名Anti-STAT3 (phospho S727) antibody
    STAT3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to STAT3 (phospho S727)
  • 特異性The antibody is blocked in Western blot by STAT3 phospho S727 peptide and partially blocked by unmodified STAT3 peptide (there is <30% cross reactivity with unmodified STAT3 as determined by ELISA).
  • アプリケーション適用あり: ICC/IF, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human STAT3 aa 700 to the C-terminus (phospho S727) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P40763

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: A431; HeLa; NIH3T3.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IHC-FoFr 1/300.

ターゲット情報

  • 機能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 関連疾患Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 配列類似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
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    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 (phospho S727) antibody 画像

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab86430) at 1/250 dilution

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) EGF Stimulated Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising Peptide at 1/250 dilution
    Lane 5 : A431 (Human epithelial carcinoma cell line) EGF Stimulated Whole Cell Lysate with Immunising Peptide at 1/250 dilution
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising Peptide at 1/250 dilution
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1/250 dilution
    Lane 8 : A431 (Human) Whole Cell Lysate (ab52255) with Non-Modified Control Peptide at 1/250 dilution
    Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1/250 dilution

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Anti-STAT3 (phospho S727) antibody (ab86430) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 180 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab86430 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86430 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde (4%, 10min) fixed cells at 1ug/ml, and also in HeLa, Hek293, HepG2, and MCF-7 Methanol (100%, 5min) fixed cells at 1ug/ml.

  • IHC- FoFr image of STAT3 (phospho S727) staining using rat hypothalamus at low (left) and high (right) magnification. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

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Anti-STAT3 (phospho S727) antibody (ab86430) 使用論文

This product has been referenced in:
  • Mishra V  et al. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ 7:16 (2016). Mouse . Read more (PubMed: 26949510) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4–15%)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 11 2014


I am very pleased to hear you would like to accept our offer and test ab86430 in sheep. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for sheep and include this code in th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain - Hypothalamus)
Specification Brain - Hypothalamus
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Mar 20 2012

Thank you for contacting us.  Both ab86132 and ab86430 are raised in rabbit and bind to the phosphorylated serine 727 in STAT1 and STAT3 respectively.  I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"