製品の概要
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製品名Anti-STAT3 (phospho S727) antibody
STAT3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to STAT3 (phospho S727)
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由来種Rabbit
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特異性The antibody is blocked in Western blot by STAT3 phospho S727 peptide and partially blocked by unmodified STAT3 peptide (there is <30% cross reactivity with unmodified STAT3 as determined by ELISA).
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アプリケーション適用あり: ICC/IF, WB, IHC-FoFrmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human STAT3 aa 700 to the C-terminus (phospho S727) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P40763 -
ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: A431; HeLa; NIH3T3.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab86430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | 1/250. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa). | |
| IHC-FoFr | 1/300. |
ターゲット情報
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機能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
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組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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関連疾患Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
Autoimmune disease, multisystem, infantile-onset -
配列類似性Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
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細胞内局在Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
- Information by UniProt
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参照データベース
- Entrez Gene: 508541 Cow
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 733648 Pig
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P61635 Cow
- SwissProt: P40763 Human
see all -
別名
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
see all
画像
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Western blot - Anti-STAT3 (phospho S727) antibody (ab86430)All lanes : Anti-STAT3 (phospho S727) antibody (ab86430) at 1/250 dilution
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) EGF Stimulated Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising Peptide at 1/250 dilution
Lane 5 : A431 (Human epithelial carcinoma cell line) EGF Stimulated Whole Cell Lysate with Immunising Peptide at 1/250 dilution
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising Peptide at 1/250 dilution
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1/250 dilution
Lane 8 : A431 (Human) Whole Cell Lysate (ab52255) with Non-Modified Control Peptide at 1/250 dilution
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-Modified Control Peptide at 1/250 dilution
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Western blot - Anti-STAT3 (phospho S727) antibody (ab86430)Anti-STAT3 (phospho S727) antibody (ab86430) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 180 kDa, 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-STAT3 (phospho S727) antibody (ab86430)ICC/IF image of ab86430 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86430 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde (4%, 10min) fixed cells at 1ug/ml, and also in HeLa, Hek293, HepG2, and MCF-7 Methanol (100%, 5min) fixed cells at 1ug/ml.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-STAT3 (phospho S727) antibody (ab86430)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, FranceIHC- FoFr image of STAT3 (phospho S727) staining using rat hypothalamus at low (left) and high (right) magnification. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
プロトコール
参考文献
This product has been referenced in:
- Mishra V et al. Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation. Biol Sex Differ 7:16 (2016). Mouse . Read more (PubMed: 26949510) »
- Cabello N et al. Sex differences in the expression of lung inflammatory mediators in response to ozone. Am J Physiol Lung Cell Mol Physiol 309:L1150-63 (2015). WB ; Mouse . Read more (PubMed: 26342085) »
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